血清沉淀常见问题

 
血清沉淀常见问题
 
 
 

血清中经常会出现肉眼可见的沉淀,其成分有多种类型,产生的原因有很多,但这并不会影响血清的品质。目前市面上所有品牌的血清产品均存在这一现象 [1-3]。

 
问题一

血清沉淀是什么?

 

Figure 1. 血清因反复冻融而造成纤维蛋白原的凝集

问题二

血清沉淀会影响细胞培养吗?

 

问题三

应该怎样操作以尽量避免沉淀出现?

 

问题四
出现血清沉淀该如何处理?

 

 

 

 
 
总结
 
 

血清产品中出现沉淀属于常见现象,无需过多担心,并且不会影响血清的品质,对细胞培养而言也是没有任何问题的,您可以放心使用。

 

参考文献:

[1] THEMOFISHER. Why does material precipitate in FBS upon storage in the refrigerator? [M]. Product FAQs.

URL:https://www.thermofisher.com/search/supportSearch?query=+precipitate+serum+&navId=4294959582%2B4294959572.

[2] ATCC. Turbidity and precipitates in serum [M]. FAQs. URL: https://www.atcc.org/support/faqs/f4ba3/Turbidity and precipitates in serum-67.aspx.

[3] GEMINI. Precipitates in Serum [M]. Technical Topics of Interest. URL: http://www.gembio.com/post/precipitates-serum.

[4] KELL D B, PRETORIUS E. The simultaneous occurrence of both hypercoagulability and hypofibrinolysis in blood and serum during systemic inflammation, and the roles of iron and fibrin(ogen) [J]. Integr Biol (Camb), 2015, 7(1): 24-52.

[5] RAOULT D, DRANCOURT M, AZZA S, et al. Nanobacteria are mineralo fetuin complexes [J]. Plos Pathogens, 2008, 4(2):

[6] SCHLIEPER G, KRUGER T, HEISS A, et al. A red herring in vascular calcification: 'nanobacteria' are protein-mineral complexes involved in biomineralization [J]. Nephrol Dial Transplant, 2011, 26(11): 3436-3439.

[7] Nanobacteria may only be nanoparticles [J]. Chemical & Engineering News, 2008, 86(14): 51-51.

 

 

 

 

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