从肿瘤组织到活细胞（肿瘤侵润性淋巴细胞）制备完整手册

样品的采集和获得

样品储存条件优化的必要性

Part.1

在样本收集过程中，经常需要在多个不同的时间点收集多种器官的组织在样本储存过程中避免细胞坏死和凋亡，特别是需要防止干细胞功能的丢失，最长时间48h。

免疫细胞在储存过程中可能被活化而导致下游实验分析的偏差。

优势

Part.2

方便：即用型产品，专为储存新鲜器官和组织而研发设计；
经过多种组织的测试（肿瘤、皮肤、心脏、脑、脾脏、骨骼肌）无抗生素或抗菌素；
不会引起组织的细胞活化或凋亡，避免背景干扰。
创新性：独特的配方，让您短时间内解离得到高活力的细胞（大量文献信息）
轻柔：对细胞损伤小，充分保护细胞膜的完整性及分子表位的完好（如需要可以咨询我们索取参数表）
重复性好：标准化的方法和流程，保证了结果的可重复性

应用

Part.3

MACS组织储存液专门优化用于储存新鲜组织和器官，而不会介导细胞活化或凋亡。该试剂已经在人和小鼠的各种器官（包括肿瘤，皮肤，心脏，脾脏，脑和骨骼肌等组织）中试用过，效果甚佳。

订购信息

订购信息：

MACS®组织解离试剂盒：

许多下游实验（包括抗体结合与识别）都要求解离到的细胞活力好、膜完整、表位完好。MACS组织解离试剂盒就是为此设计的，让您高效、轻柔地解离多种组织样品。

该试剂盒与gentleMACS Dissociator 或gentleMACS Octo Dissociator配合，结合优化了的酶消化和机械剪切处理，让您短时间内得到高活力的单细胞悬液。

为了更加便捷、标准化地进行组织解离和匀浆，美天旎研发了自动化组织处理器——gentleMACS Dissociator Instruments 。

快捷：全自动地组织解离或匀浆；
稳定：标准化步骤，结果可靠，可重复性高；
灵活：预设程序及用户可自定义的程序，几乎可以处理所有类型的组织。

gentleMACS Dissociator ——两通道组织处理器：自带多个预设程序，半自动化的流程，可以处理多种类型的组织样本，为您提供标准化和可靠的组织解离和匀浆效果。
gentleMACS Octo Dissociator ——八通道组织处理器：自带多种预设程序，且用户可以根据需要自定义程序，几乎可以处理所有类型的组织。另外，其容量大，可同时处理8个样本，且可以同时运行不同的程序。
gentleMACS Octo Dissociator with Heaters ——带加热模块的八通道组织处理器：8个通道，分别带有独立的加热模块，可以进行全自动的组织解离。同样，该仪器自带预设程序，也可以自定义程序，因此可以处理几乎所有类型的组织。

gentleMACS相关的文献已经超过万篇，如您有兴趣欢迎联系我们，我们可以为您安排相关的demo演示机会。

MACS SmartStrainers（30um 70um 100um）方便我们去除样品制备过程中的细胞团块且不容易堵塞。

通过gentleMACS Dissociator 获取的细胞可以满足后续的分选培养的实验需要。

肿瘤侵润性淋巴细胞：CD137 is mainly expressed on activated CD4+ and CD8+ T cells, activated B cells, and on natural killer cells, but can also be found on resting monocytes and dendritic cells. As a costimulatory molecule CD137 is involved in the activation and survival of CD4+ or CD8+ T cells, and NK cells.

利用cd137MicroBead Kit 可以从肿瘤样品里面分离得到cd137+目的细胞。

移植瘤细胞纯化利器：Mouse Cell Depletion Kit Mouse Cell Depletion Kit has been designed for the enrichment of untouched human cells upon xenotransplantation. Human tumor xenografts represent the benchmark for research areas like drug discovery, cancer stem cell biology, and metastasis prediction1. During the growth phase in vivo, xenografted tissue is vascularized and infiltrated by cells of mouse origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells. The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, and region of transplantation. However, even when these factors are kept constant, the amount and composition of infiltrating mouse cells are highly variable, which makes accurate molecular downstream analyses difficult. The contaminating mouse cells lead to cross-hybridization of mouse-derived molecules to human probes on microarrays and a significant reduction of sensitivity caused by measurement of mouse signals during next-generation sequencing or proteome analysis2. In addition, the culture of human tumor cells is frequently hampered by murine fibroblasts overgrowing the target cells.

Figure 2

EpCAM positive human colon carcinoma cells were isolated from a heterogenous cell population and subsequently, the unseparated fraction, the human tumor cell fraction, and the mouse cell containing fraction were cultured for three days in expansion medium (90% RPMI-1640, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for murine vimentin (red) and human EpCAM (green). Cell nuclei were stained with DAPI (blue).

Unseparated fraction

Isolated human tumor cells